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The following day, sections were rinsed with PBS and then incubated for 3 h at 2225 C with Cy3-conjugated anti-mouse (Jackson ImmunoResearch, 715-165-150) at 1:500 dilution in a solution of 1.5% BSA in PBS, respectively

Slowly breathe out through your mouth for a count of 7

The qPCR was run using total cDNA combined with AceQ SYBR qPCR Master Mix (Q111-03, Vayzme, Nanjing, China) and primers (Supplementary Table 2) on a Bio-Rad thermal cycler